rabbit polyclonal anti cleaved drosophila dcp1  (Cell Signaling Technology Inc)

Journal: EMBO Reports

Article Title: Toll signaling controls stem cell proliferation in intestinal regeneration and tumorigenesis

doi: 10.1038/s44319-026-00693-9

Figure Lengend Snippet: ( A ) Representative images of the posterior midgut of flies with indicated genotypes at 29 °C for 16 days. PH3 staining (red), Nuclei (blue), and esg > GFP (green). Scale bar: 100 μm. ( B ) Representative images of the posterior midgut of flies with indicated genotypes at 29 °C for 16 days. PH3 staining (red), Nuclei (blue), and esg > GFP (green). Scale bar: 100 μm. ( C ) Representative images of the posterior midgut of flies with indicated genotypes at 29 °C for 16 days. TUNEL staining (red), Nuclei (blue), and esg > GFP (green). Scale bar: 50 μm. ( D ) Quantification of TUNEL-positive cells in the posterior midgut of flies with the indicated genotypes after 16 days at 29 °C. n = 15, 11, 12, 13, 12 (from left to right). ( E ) Representative images of the posterior midgut of flies with indicated genotypes at 29 °C for 16 days. DCP1 staining (red), Nuclei (blue), and esg > GFP (green). Scale bar: 50 μm. ( F ) Representative images of the posterior midgut of flies with indicated genotypes at 29 °C for 8 days. p-Akt staining (red), Nuclei (blue), and esg > GFP (green). Scale bar: 10 μm. Data information: In ( D ), box plots indicate median (center line), 25th–75th percentiles (box) and minima/maxima within 1.5 × interquartile range (whiskers); outliers are shown as individual points. Statistical significance in ( D ) was determined using a two-tailed unpaired t -test ( ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001). Exact p values are provided in Table .

Article Snippet: Rabbit polyclonal anti-cleaved Drosophila Dcp1 (Asp215) , Cell Signaling Technology , Cat#9578S.

Techniques: Staining, TUNEL Assay, Two Tailed Test

Journal: PLOS Biology

Article Title: Activation of the Drosophila innate immune system accelerates growth in cooperation with oncogenic Ras

doi: 10.1371/journal.pbio.3003068

Figure Lengend Snippet: (A) Confocal images of EADs and tumors of indicated genotypes at 96-h AED labeled for Dcp1 illustrating the enrichment of cell death after Dorsal overexpression in control EADs as quantified in (B) and demonstrating the loss of cell death after inhibition of apoptosis through overexpression of Ras V12 or p35. For simplicity, all genotypes with overexpression of p35 are only shown with a nuclear marker (gray); a quantification of the Dcp1-positive area can, however, be found in S4F Fig. The white outline highlights the GFP-marked region. (B) Quantification of the Dcp1 positive area relative to whole disc size at 96-h AED. Mean area and standard deviation (error bar) are shown (*** p < 0.001, One-way ANOVA with post-hoc Tukey HSD). (C) qRT-PCR analysis of rpr mRNA levels in EADs expressing indicated UAS-transgenes under the control of an eye-specific Gal4 ( eyFlp; act>CD2>Gal4 ) illustrating the increase in pro-apoptotic gene expression after overexpression of Dorsal in control EADs. Data is shown as fold changes relative to the control (lacZ). n = 3 or n = 4 biologically independent samples were analyzed. Median fold changes and interquartile ranges (error bars) are shown. The dashed horizontal line illustrates the reference fold change of 1. (D) Quantification of tissue size of indicated genotypes at 96-h AED to illustrate changes to the effects of Dorsal on tissue growth after inhibition of apoptosis through co-expression of p35. Mean area and standard deviation (error bar) are shown (*** p < 0.001, One-way ANOVA with post-hoc Tukey HSD). (E) Graphical model illustrating the transition of the function of NF-κB/ Dorsal from growth-suppressive during development to growth-promoting during Ras V12 -mediated oncogenesis. The transition is mediated by the combined effects of Dorsal-induced changes in cell death, cell survival, proliferation and differentiation. In all confocal images, DAPI (gray) is used to visualize nuclei and GFP (green) labels cells co-expressing indicated UAS-transgenes. The underlying data sets can be found in . Scale bars represent 100 µm. EAD, eye-antennal disc; AED, after egg deposition; Dl, Dorsal; Dcp1, cleaved Drosophila caspase 1; rpr, reaper; NF-κB, nuclear factor ‘kappa-light-chain-enhancer’ of activated B-cells.

Article Snippet: rabbit polyclonal anti-cleaved Dcp1 , Cell Signaling Technologies , AB_2721060 , 1:200.

Techniques: Labeling, Over Expression, Control, Inhibition, Marker, Standard Deviation, Quantitative RT-PCR, Expressing, Gene Expression

Journal: PLOS Biology

Article Title: Activation of the Drosophila innate immune system accelerates growth in cooperation with oncogenic Ras

doi: 10.1371/journal.pbio.3003068

Figure Lengend Snippet: Primary antibodies used in this study.

Article Snippet: rabbit polyclonal anti-cleaved Dcp1 , Cell Signaling Technologies , AB_2721060 , 1:200.

Techniques:

Journal: eLife

Article Title: Cell competition removes segmental aneuploid cells from Drosophila imaginal disc-derived tissues based on ribosomal protein gene dose

doi: 10.7554/eLife.61172

Figure Lengend Snippet:

Article Snippet: Antibody , Polyclonal Rabbit anti-active-Dcp1 , Cell Signalling Technology , Cat #9578 , (1:50) dilution.

Techniques: Recombinant, Plasmid Preparation